Introduction
Methods
Eligibility criteria
Search strategy
Study selection
Data extraction
Risk of bias assessment
Statistical analysis
Results
Literature search results
Description of studies
Author, country, year | Study design | Population | Age | TBSA | Xenograft type | Intervention group | Control group | Main findings |
---|---|---|---|---|---|---|---|---|
Bovine xenografts | ||||||||
Tuleubayev et al. [25], Kazakhstan 2022 | RCT | 68 patients (47 male and 21 female patients) | 13.13 ± 5.03% in DBP and 12.11 ± 6.54% in control group | 2A Grade burns in both groups | Decellularized bovine peritoneum (DBP) | DBP (31 patients) | Dressings impregnated with 10% Povidone–Iodine (37 patients) | Hospitalization: 10.45 ± 6.15 (DBP) vs. 9.92 ± 6.08 (control) (Not significant) Dressing change: 1.35 ± 0.66 (DBP) vs. 5.22 ± 3 (control) Level of pain: Significantly lower in DBP group Re-epithelialization: 23 of 31 and 24 of 37 patients (not significant) |
Porcine xenografts | ||||||||
Feng et al. [22], China 2006 | RCT | 40 patients (20 patients in porcine ADM group and 20 in control group) | 6 months–88 years | 30–94% | Xenogenic ADM (porcine) | Porcine ADM overlapped 2–3 cm at the junctions of separate pieces | Topical antimicrobial agents (povidone–iodine ointment) three times daily and the wound was exposed | Healing time: 9–14 days (porcine) vs. 14–35 days (control) Scar index (3 months): 3.29 ± 1.63 (porcine) vs. 7.75 ± 1.78 (control) Scar index (2 years): 2.77 ± 1.05 (porcine) vs. 7.03 ± 1.24 (control) Dressing changes: No dressing change (porcine) |
Zuo et al. [26], China 2016 | RCT | 6 adult burn patients (4 male and 3 female patients) | 24.8 (18–35) years | 88.3% ± 5.7% (total burn areas) and 81.6% ± 7.8% (full thickness burn areas) | Porcine xenograft | Fresh pigskins in 2 patients (7/15 operations) | Partial-thickness viable cryopreserved alloskins in 4 patients (8/15 operations) | Autoskin grafted time: 27.3 ± 3.8 days (1st operation), 22.0 ± 5.7 days (2nd operation), and 15.3 ± 1.5 days (3rd operation) Survival percentage: POW 1: 80.0% ± 10.0% (alloskin) vs. 75.7% ± 5.3% (pigskin) (P = 0.16) POW 2: 71.2% ± 10.6% (alloskin) vs. 66.4% ± 6.2% (pigskin) (P = 0.30) POW 3: 48.7% ± 2.5% (alloskin) vs. 35.0% ± 7.0% (pigskin) (P = 0.03) TBSA that survived: 21.8% ± 10.9% (alloskin) vs. 22.4% ± 8.5% (pigskin) |
Chen et al. [27], China 2013 | RCT | 30 patients (20 males and 10 females) | 18–60 years | 25–60% | Porcine acellular dermal xenograft (ADX) | ADX and split-thickness skin autograft | Split-autologous epidermal skin | Vancouver Scar Scale: Not significant after 1 month but significant after 3, 6, and 12 months Adverse reaction: No ulcer or scar hyperplasia |
Zajicek et al. [24], Czech Republic 2011 | RCT | 86 pediatric patients with superficial scald burns in 2 groups | 5 months–7 years | 1–35% in total; 10 (6–13) % in Xe-Derma and 7 (4–10) % in Askina THINSite (P = 0.028) | Acellular pig dermis Xe-Derma (porcine) | Xe-Derma (43 patients) | Synthetic hydrogel wound dressing Askina THINSite (43 patients) | Re-epithelialization: 8 (5–10) days (Xe-Derma) vs. 7 (3–10) days (Askina THINSite) (P = 0.147) Infection: 6 (Xe-Derma) vs. 10 (Askina THINSite) (p = 0.2) Dressing changes: One on day 2 or 3 and outer dressing change every 2 or 3 days |
Karlsson et al. [23], Sweden 2022 | RCT | 24 patients (22 male and 2 female patients) | 39 (19–73) years | 11 (4–37) % in total; 7 (2–14) % treated with dressings | Porcine xenograft (EZ derm) | EZ derm (11 patients) | Biosynthetic cellulose dressing (BsC) (13 patients) | Healing time: 19 (12–35) in porcine vs. 18 (10–35) in BsC (P = 0.7) Hospital stay: 14 (2–28) days in porcine vs. 4 (0–40) days in BsC (P = 0.331) Patients’ POSAS total score (12-month follow-up): 45 (31–61) (porcine) vs. 33 (11–55) (BsC) (P = 0.39) Observer POSAS total score (12-month follow-up): 20 (13–40) (porcine) vs. 19 (11–25) (BsC) (p = 0.45) Infection: 11 of 11 (porcine) vs. 12 of 13 (BsC) (p = 1.0) |
Hosseini et al. [28], Iran 2008 | non-randomized clinical trial | 86 burned pediatrics | 4 (0.1–15) years | 28.8 (10–50) % in conventional and 28.2 (10–54) % in Xenoderm group | Xenoderm: lyophilized pig skin | Xenoderm (51 patients) | Conventional treatments (SSD) (35 patients) | Hospital stay in all patients: 10 (10.8) in Xenoderm vs. 17 (14.4) days (conventional) (p = 0.10) Hospital stay in TBSA of 20–39%: 7.5 (3–36) days (Xenoderm) vs. 20 (4–55) days (conventional) (p = 0.001) Dressing changes: 6.02 (8.3) (Xenoderm) vs. 12.9 (9.3) times (conventional) (p = 0.0005) Mortality: 0 (Xenoderm) vs. 5 (conventional) |
Hosseini et al. [29], Iran 2009 | non-randomized clinical trial | 118 burn patients | 26.54 (2–80) in conventional and 26.52 (1–81) years in Xenoderm group | 30–75%; 44.7 (31–70) % in conventional and 42.8 (30–72) % in Xenoderm group | Xenoderm: lyophilized pig skin | Xenoderm (65 patients) | Conventional treatment (saline-soaked dressing) (53 patients) | Hospital stay: 18.7 (15.2) (Xenoderm) vs. 24.2 (18.2) days (conventional) (p = 0.11) Dressing changes: 10.4 (10.9) (Xenoderm) vs. 18.04 (13.6) (conventional) (P = 0.005) Mortality: 7 (10.8%) (Xenoderm) vs. 19 (35.8%) (conventional) (P = 0.001) |
Fish xenografts | ||||||||
Li et al. [30], 2021 | animal study | 30 Sprague–Dawley rats and 5 Bama mini-pigs | 6–8-week-old rats | NA (3 round full-thickness skin defects with a diameter of 1.8 cm on each rat and six 5 × 5 cm2 square full-thickness skin defect on each pig) | Fish skin-derived ADM (TS-ADM) and porcine ADM (DC-ADM) | TS (alkaline decellularization and γ-irradiation sterilization without freeze-drying) | DC (porcine ADM as active control) and Vaseline gauze (VLGZ as negative control) | Re-epithelialization (2w) in pigs: 23.4% ± 6.3% in TS vs. 10.7% ± 2.6% in VLGZ vs. 12.4% ± 4.6% in DC (significant) Wound dressing: after 2 weeks, TS was significantly easier to remove Wound closure rate (day 35): TS inhibited scar hyperplasia; collagen deposition in group TS was notably higher than other two groups |
Lima et al. [31], Brazil 2020 | Phase II Pilot RCT | 30 pediatrics | 2–12 years | < 20% | Tilapia skin (TS) | Tilapia skin (TS) | SSD cream 1% | Re-epithelialization: 10.07 ± 0.46 (TS) vs. 10.47 ± 0.74 days (SSD) Dressing changes: 3.00 ± 0.76 (TS) vs. 9.27 ± 1.39 (SSD) Anesthetic use: lower in TS group |
Lima et al. [15], Brazil 2020 | Phase II RCT | 62 patients (Arm A: SPTB involving < 10% of TBSA; Arm B: SPTB involving 10–20% of TBSA; Arm C: DPTB involving 5–15% of TBSA) | 18–50 years | SPTB affecting up to 20% TBSA, or DPTB affecting 5–15% TBSA | Nile Tilapia Fish Skin (NTFS) | NTFS (A: 13, B: 9, and C: 10) | SSD cream 1% group (A: 10, B: 10, and C: 10) | Re-epithelialization: NTFS (A: 9.77 ± 0.83; B: 10.56 ± 1.13; C: 18.10 ± 0.99) and SSD (A: 11.20 ± 0.063; B: 11.70 ± 0.067; C: 21.30 ± 1.42) [significant] Dressing changes: NTFS (A: 2.08 ± 0.28; B: 2.33 ± 0.71; C: 6.10 ± 2.02) and SSD (A: 5.80 ± 0.42; B: 11.00 ± 0.47; C: 20.20 ± 1.69) [significant] |
Lima et al. [32], Brazil 2021 | Phase III RCT | 115 outpatients with SPTB | 18–70 years | < 15% | Nile Tilapia Fish Skin (NTFS) | Glycerolized fish skin (NTFS) (57 patients) | SSD cream 1% (58 patients) | Re-epithelialization: 9.7 ± 0.6 in NTFS vs. 10.2 ± 0.9 in SSD (P = 0.001) Dressing changes: 1.6 ± 0.7 in NTFS vs. 4.9 ± 0.5 in SSD (P < 0.001) |
Stone et al. [33], USA 2018 | animal study | 36 full thickness burn wounds on pigs | NR | NA | omega-3 rich fish skin graft (FSG) | A) FSG (day 0) + 1.5:1 mSTSG (day 7); B) FSG (day 0) + 3:1 mSTSG and FSG applied over the graft (day 7) C) cadaver porcine skin (day 0) + 1.5:1 mSTSG (day 7); | Infection: No infection in FSG Outcome measures, including contraction rates, TEWL measurements, hydration levels, and blood perfusion levels: FSG was similar to cadaver skin Wound healing: The 3:1 mSTSG treated with FSG resulted in similar healing as the wounds treated with the 1.5:1 mSTSG | |
Stone et al. [34], USA 2021 | animal study | 6 female Yorkshire pigs (24 deep partial-thickness and 36 full-thickness burns) | NR | NA | Fish skin graft (FSG) or fetal bovine dermis (FBD) | FSG | FBD | Re-epithelialization (day 14): 50.2% in FSG vs. 23.5% in FBD (P < 0.005) Reduction in original wound size (day 14): 93.1% in FSG vs. 106.7% in FBD (P = 0.005) |